Due to the high hydrophobicity of the PVDF membrane, it needs to be soaked in an appropriate solution such as ethanol before use to achieve the purpose of activation and make the membrane easier to bind proteins. This product is compatible with many dyes, such as Ponceau-S red, Coomassie brilliantblue, Sypro ruby, Transillumination, Toluidine blue, CPTS, Colloidal gold, India inkAmido black. Among them, after dyeing with dyes such as Ponceau and CPTS, the dyes can be washed off and the film can be used for further analysis; while dyes such as Coomassie Brilliant Blue, India ink, and amide black are irreversible, the film cannot be used after dyeing. For further analysis.